The Effects of Histaminergic Agents in the Nucleus Accumbens of Rats in the Elevated Plus-Maze Test of Anxiety
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Abstract
Objective: The nucleus accumbens (NAc) receive histaminergic neurons from tuberomammillary nuclei. There are reports indicating that central histamine systems are involved in many physiological behavioral processes, including anxiety. The aim of the present study was to assess whether the histaminergic system of the NAc is involved in anxiety-related behaviors.
Methods: Rats were anesthetized with intra-peritoneal injection of
ketamine hydrochloride, plus xylazine and then were placed in a stereotaxic apparatus. In addition, two stainless-steel cannuale were placed 2 mm above the nucleus accumbens shell. Seven days after recovery from surgery, the behavioral testing was started. As a model of anxiety, the elevated plus maze which is a useful test to investigate the effects of anxiogenic or anxiolytic drugs in rodents, was used in male Wistar rats.
Results: Intra-NAc administration of histamine (0.01, 0.1 and 1 µg/rat) increased the percentage of open arm time (%OAT) and open arm entries (%OAE) ,but not locomotor activity, indicating an anxiolytic response.
Furthermore, bilateral microinjections of different doses of the H1 receptor antagonist pyrilamine (0.001, 0.01, 0.1 and 1 µg/rat) or the H2 receptor antagonist ranitidine (0.001, 0.01, 0.1 and 1 µg/rat) into the NAc increased
%OAT and %OAE , but not locomotor activity. However, both histamine and histamine receptor antagonists showed an anxiolytic-like effect ; the antagonists (1 µg/rat) also decreased the histamine response.
Conclusion: The results may indicate a modulatory effect for the H1 and H2 histamine receptors of nucleus accumbens in the anxiety behavior of rats.
Brown RE, Stevens DR, Haas HL. The physiology of brain histamine. Prog Neurobiol 2001;63:637-672.
Onodera K, Miyazaki S, Imaizumi M, Stark H, Schunack W. Improvement by FUB 181, a novel histamine H3-receptor antagonist, of learning and memory in the elevated plus- maze test in mice. Naunyn Schmiedebergs Arch Pharmacol 1998;357:508-513.
Khalilzadeh A, Zarrindast MR, Djahanguiri B. Effects of intracerebroventricular administration of ultra low doses of histaminergic drugs on morphine state- dependent memory of passive avoidance in mice. Behav Brain Res 2006;166:184-187.
Zimmermann P, Privou C, Huston JP. Differential sensitivity of the caudal and rostral nucleus accumbens to the rewarding effects of a H1-histaminergic receptor blocker as measured with place-preference and self- stimulation behavior. Neuroscience 1999;94:93-103.
Ikarashi Y, Yuzurihara M. Experimental anxiety induced by histaminergics in mast cell- deficient and congenitally normal mice. Pharmacol Biochem Behav 2002;72:437-441.
Dere E, De Souza-Silva MA, Spieler RE, Lin JS, Ohtsu H, Haas HL, et al. Changes in motoric, exploratory and emotional behaviours and neuronal acetylcholine content and 5-HT turnover in histidine decarboxylase-KO mice. Eur J Neurosci 2004;20:1051-1058.
Privou C, Knoche A, Hasenohrl RU, Huston JP. The H1- and H2-histamine blockers chlorpheniramine and ranitidine applied to the nucleus basalis magnocellularis region modulate anxiety and reinforcement related processes. Neuropharmacology 1998;37:1019-1032.
Malmberg-Aiello P, Ipponi A, Bartolini A, Schunack W. Mouse light/dark box test reveals anxiogenic-like effects by activation of histamine H1 receptors. Pharmacol Biochem Behav 2002;71:313-318.
Zarrindast MR, Moghadam AH, Rostami P, Roohbakhsh A. The effects of histaminergic agents in the central amygdala of rats in the elevated plus-maze test of anxiety. Behav Pharmacol 2005;16:643-649.
Yoshitomi I, Itoh Y, Oishi R, Saeki K. Brain histamine turnover enhanced by footshock. Brain Res 1986 ;362:195-198.
Shoblock J, O'Donnell P. Histamine modulation of nucleus accumbens neurons. Ann N Y Acad Sci 2000;909:270-272.
Leurs R, Traiffort E, Arrang JM, Tardivel- Lacombe J, Ruat M, Schwartz JC. Guinea pig histamine H1 receptor. II. Stable expression in Chinese hamster ovary cells reveals the interaction with three major signal transduction pathways. J Neurochem 1994 ;62:519-527.
Traiffort E, Vizuete ML, Tardivel-Lacombe J, Souil E, Schwartz JC, Ruat M. The guinea pig histamine H2 receptor: gene cloning, tissue expression and chromosomal localization of its human counterpart. Biochem Biophys Res Commun 1995 ;211:570-577.
Hegstrand LR, Kanof PD, Greengard P. Histamine-sensitive adenylate cyclase in mammalian brain. Nature 1976;260:163-165.
Prast H, Fischer HP, Prast M, Philippu A. In vivo modulation of histamine release by autoreceptors and muscarinic acetylcholine receptors in the rat anterior hypothalamus. Naunyn Schmiedebergs Arch Pharmacol 1994;350:599-604.
Yanai K, Son LZ, Endou M, Sakurai E, Nakagawasai O, Tadano T, et al. Behavioural characterization and amounts of brain monoamines and their metabolites in mice lacking histamine H1 receptors. Neuroscience 1998;87:479-487.
Alvarez EO, Banzan AM. Histamine in dorsal and ventral hippocampus. II. Effects of H1 and H2 histamine antagonists on exploratory behavior in male rats. Physiol Behav 1986;37:39-45.
Zarrindast MR, Torabi M, Rostami P, Fazli- Tabaei S. The effects of histaminergic agents in the dorsal hippocampus of rats in the elevated plus-maze test of anxiety. Pharmacol Biochem Behav 2006 ;85:500-506.
Rostami P, Hajizadeh-Moghaddam A, Zarrindast MR. The effects of histaminergic agents in the ventral hippocampus of rats in the plus-maze test of anxiety-like behaviours. Physiol Behav 2006 ;87:891-896.
Orofino AG, Ruarte MB, Alvarez EO . Exploratory behaviour after intra-accumbens histamine and/or histamine antagonists injection in the rat. Behav Brain Res 1999;102:171-180.
Santos NR, Huston JP, Brandao ML. Blockade of histamine H2 receptors of the periaqueductal gray and inferior colliculus induces fear-like behaviors. Pharmacol Biochem Behav 2003;75:25-33.
Paxinos G, Watson C. The rat brain, in stereotaxic coordinates, 2th ed. San Diego: Academic Press ;1997.
Ahn DK, Kim KH, Ju JS, Kwon S, Park JS. Microinjection of arginine vasopressin into the central nucleus of amygdala suppressed nociceptive jaw opening reflex in freely moving rats. Brain Res Bull 2001;55:117-1121.
Rodgers RJ, Johnson NJ. Factor analysis of spatiotemporal and ethological measures in the murine elevated plus-maze test of anxiety. Pharmacol Biochem Behav 1995;52:297-303.
Pellow S, Chopin P, File SE, Briley M. Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat. J Neurosci Methods 1985;14:149-167.
Inagaki N, Yamatodani A, Ando-Yamamoto M, Tohyama M, Watanabe T, Wada H. Organization of histaminergic fibers in the rat brain. J Comp Neurol 1988;273:283-300.
Watanabe T, Taguchi Y, Shiosaka S, Tanaka J, Kubota H, Terano Y, et al. Distribution of the histaminergic neuron system in the central nervous system of rats; a fluorescent immunohistochemical analysis with histidine decarboxylase as a marker. Brain Res 1984;295:13-25.
Schwartz JC, Arrang JM, Garbarg M, Pollard H, Ruat M. Histaminergic transmission in the mammalian brain. Physiol Rev 1991;71:1-51.
Steinbusch HW, Sauren Y, Groenewegen H, Watanabe T, Mulder AH. Histaminergic projections from the premammillary and posterior hypothalamic region to the caudate- putamen complex in the rat. Brain Res 1986;368:389-393.
Chronister RB, Palmer GC, Defrance JF, Sikes RW, Hubbard JI. Histamine: correlative studies in nucleus accumbens. J Neurobiol 1982;13:23-37.
Ruarte MB, Orofino AG, Alvarez EO. Hippocampal histamine receptors and conflictive exploration in the rat: studies using the elevated asymmetric plus-maze. Braz J Med Biol Res 1997;30:1451-1461.
Alvarez EO, Ruarte MB, Banzan AM. Histaminergic systems of the limbic complex on learning and motivation. Behav Brain Res 2001;124:195-202.
Bannerman DM, Rawlins JN, McHugh SB, Deacon RM, Yee BK, Bast T, et al. Regional dissociations within the hippocampus--memory and anxiety. Neurosci Biobehav Rev 2004;28:273-283.
Schwartz JC, Arrang JM, Garbarg M, Korner M. Properties and roles of the three subclasses of histamine receptors in brain. J Exp Biol 1986;124:203-224.
Schlicker E, Malinowska B, Kathmann M, Gothert M. Modulation of neurotransmitter release via histamine H3 heteroreceptors. Fundam Clin Pharmacol 1994;8:128-137.
Yanai K, Son LZ, Endou M, Sakurai E, Watanabe T. Targeting disruption of histamine H1 receptors in mice: behavioral and neurochemical characterization. Life Sci 1998;62:1607-1610.
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| Issue | Vol 5 No 1 (2010) | |
| Section | Articles | |
| Keywords | ||
| Anxiety Histamine Maze learning Pyrilamine Ranitidine Rats | ||
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